Characteristics of mouse embryonic hepatic stem cells in different embryodurations

doi:10.3969/j.issn.2095-4344.2013.23.014

Abstract

Abstract:

BACKGROUND: Increasing attention has been paid on embryonic hepatic stem cells that have stronger proliferation and differentiation capacities, as well as lower immunogenicity and immunological activity than bone marrow stem cells. The majority of related studies focus on adult hepatic stem cells, and little evidence addresses mouse embryonic hepatic stem cells.
OBJECTIVE: To compare the biological characteristics of mouse embryonic hepatic stem cells at different gestational ages and to explore the changes of stem cells during embryonic hepatic development process.
METHODS: The mouse embryonic hepatic stem cells at different gestational ages (13.5, 16.5 and 19.5 days) were isolated and cultured with mechanical separation, enzyme digestion and differential adherence methods. The primary cell morphology, growth and phenotypic markers were observed and compared.
RESULTS AND CONCLUSION: The embryonic hepatic stem cells at gestational 13.5 days showed uniform morphology, good growth, and apparent characteristics of stem cells, the albumin and cytokeratin 19 were not expressed. This is evidence that embryonic hepatic stem cells are under primary un-differentiated stage. As the increase of gestational age, embryonic hepatic stem cells presented morphological changes and poor growth, the albumin and cytokeratin 19 expression was increased. Experimental findings indicate that, embryonic hepatic stem cells may differentiate into double dominant stem cells with both hepatic cells and biliary tract markers.

Key words: stem cells, stem cell culture and differentiation, Kunming mice, gestational age, embryonic hepatic stem cells, morphology, cell surface marker, alpha-fetoprotein, c-kit, albumin, cytokeratin 19, stem cell photographs-containing paper

CLC Number:

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Wu Bi-gang, Chang Jing, Zhang Xiao-gang. Characteristics of mouse embryonic hepatic stem cells in different embryodurations[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(23): 4279-4285.

Figures/Tables 5

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